Mountains in the Chaning World, Mountains in the Chaning World

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DEVELOPMENT OF PAPER-BASED ANALYTICAL DEVICE FOR HEAVY METAL ANALYSIS OF LEACHATE SAMPLES FROM LANDFILL SITES OF BAGMATI ZONE, NEPAL
Tshering Ongmu Sherpa, Basant Giri

Last modified: 2017-08-24

Abstract


Abstract

Currently in Nepal, there is lack of provision regarding separate handling of hazardous waste containing heavy metals. Such situation has increased the probability of heavy metals leaching into the surrounding groundwater and soil. Various studies have been conducted to detect and quantify heavy metals in groundwater and soil, with all showing exceeding levels of concentration than the standard limit which is implicating threat to public health and environment. Heavy metals are generally measured using atomic absorption spectrometer which requires expensive equipment and trained personnel. There is a need for a simple, low-cost and portable method. In this research, we aimed to develop microfluidic paper-based analytical devices using Whatman grade 1 filter paper and wax crayons. The deposited wax was later heated to form a hydrophobic barrier on the paper device. Within the barrier, the detection reagents of given metal react with the heavy metals to produce their respective color. We used visual detection using eyes for qualitative results. For quantitative results, we imaged the paper device and analyzed on ImageJ software. The custom built paper device was used to generate a response curve for nickel and iron with a calibration range of 100-1.56 mg/ml. The curves were used to measure the metals in leachate samples collected from Sisdol, Gokarna and Sundarighat. The nickel could not be quantified as it was below detection level of paper device but, the samples showed to consist a maximum of 1.09 ± 0.02 mg/ml to a minimum of 0.49 ± 0.01 mg/ml of iron.

Keywords: Paper-based analytical device, leachate, microfluidics, heavy metals, smartphone, ImageJ

 


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