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EFFECT OF NEEM EXTRACT AND ANTIBIOTICS ON INVITRO GROWTH OF Rastonia solanacearum
Manisha Poudel

Last modified: 2017-08-31

Abstract


Bacterial wilt caused by a soil borne bacterium Ralstonia solanacearum is a devastating disease attacking a number of agriculturally important crops. Various efforts have been paid to develop economically sound and environmentally safe techniques of management for this disease however such techniques are still lagging. Thus we aimed to find a cost effective and ecofriendly measures for the control of bacterial wilt. Here we have evaluated the antibacterial property of Neem extract with streptomycin and CuSO4 in paper disc inhibition method. Bacterial culture plates were prepared by spreading the bacterial suspension from 48 hours old cultures on the 523 agar plates. Treatments were applied on different spots on the paper disc kept at regular interval on the bacterial culture plates. A completely randomized experimental design was followed with 3 treatments and 7 replications. The efficiency of each treatment was compared according to the size of zone of inhibition (ZOI). Diameters of ZOI were measured after 48 hours of inoculation. Our result revealed that among these three treatments, streptomycin was able to restrict the bacterial growth with forming the largest ZOI whereas the aqueous extract of Neem and CuSO4 was unable restrict the growth of bacteria in applied concentration of 1:1 ratio by fresh weight and 0.2% respectively.

Keywords: Bacterial wilt, Neem, Streptomycin, ZOI


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